Friday, August 12, 2011
Complementary nucleotide sequences between RNA and DNA?
You can do a hybridization test. First, you boil your duplex DNA for 5 min to break the hydrogen bonds. The DNA will thus be single stranded (the 2 strands are not longer hybridizing to each other). Immediately after boiling, you put a droplet of that single stranded DNA on a special membrane. You can also put some droplets of other, boiled DNAs next to "your" DNA, as a control. Then, you label your RNA with a dye or a radioactive compound, and put a droplet of that on top of your DNA droplet(s). Then you wash it, to remove not-bound RNA. Then you dry the membrane and put an X-ray film on it. Only where the labeled RNA has bound, will you see a black dot. In that case, the RNA has a complementary match to the DNA.
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